plasmid supernova/prsetb  (Addgene inc)

Journal: Free radical biology & medicine

Article Title: Quantification of reactive oxygen species production by the red fluorescent proteins KillerRed, SuperNova and mCherry.

doi: 10.1016/j.freeradbiomed.2019.12.008

Figure Lengend Snippet: (a) Absorbance spectrum of the photosensitizers Rose Bengal dye (0.0026 mg/mL), mCherry (0.22 mg/mL), tdKillerRed (0.25 mg/mL), and SuperNova (0.76 mg/mL). See Table 1 for quantification.

Article Snippet: SuperNova/pRSETB was a gift from Dr. Takeharu Nagai (Addgene plasmid # 53234) [ 8 ]. mCherry (pmCherry-C1) and tdKillerRed (#FP963, Evrogen) were amplified and ligated into pRSETB using BamHI and EcoRI.

Techniques:

Journal: Free radical biology & medicine

Article Title: Quantification of reactive oxygen species production by the red fluorescent proteins KillerRed, SuperNova and mCherry.

doi: 10.1016/j.freeradbiomed.2019.12.008

Figure Lengend Snippet: Photosensitizer absorbance at 561 nm.

Article Snippet: SuperNova/pRSETB was a gift from Dr. Takeharu Nagai (Addgene plasmid # 53234) [ 8 ]. mCherry (pmCherry-C1) and tdKillerRed (#FP963, Evrogen) were amplified and ligated into pRSETB using BamHI and EcoRI.

Techniques:

Journal: Free radical biology & medicine

Article Title: Quantification of reactive oxygen species production by the red fluorescent proteins KillerRed, SuperNova and mCherry.

doi: 10.1016/j.freeradbiomed.2019.12.008

Figure Lengend Snippet: (a) Rose Bengal, (b) tdKillerRed, Supernova, mCherry and control (no photosensitizer) were irradiated with equal molar absorptivity at 561 nm in the presence of DHE (100 μM) for quantification of 2-OHE+. Values are mean ± SD for n = 3 independent experiments.

Article Snippet: SuperNova/pRSETB was a gift from Dr. Takeharu Nagai (Addgene plasmid # 53234) [ 8 ]. mCherry (pmCherry-C1) and tdKillerRed (#FP963, Evrogen) were amplified and ligated into pRSETB using BamHI and EcoRI.

Techniques: Irradiation

Journal: Free radical biology & medicine

Article Title: Quantification of reactive oxygen species production by the red fluorescent proteins KillerRed, SuperNova and mCherry.

doi: 10.1016/j.freeradbiomed.2019.12.008

Figure Lengend Snippet: Superoxide and singlet oxygen quantum yield of mCherry, tdKillerRed, and SuperNova.

Article Snippet: SuperNova/pRSETB was a gift from Dr. Takeharu Nagai (Addgene plasmid # 53234) [ 8 ]. mCherry (pmCherry-C1) and tdKillerRed (#FP963, Evrogen) were amplified and ligated into pRSETB using BamHI and EcoRI.

Techniques:

Journal: Free radical biology & medicine

Article Title: Quantification of reactive oxygen species production by the red fluorescent proteins KillerRed, SuperNova and mCherry.

doi: 10.1016/j.freeradbiomed.2019.12.008

Figure Lengend Snippet: (a) xanthine/xanthine oxidase (X/XO) O2•- production was assessed using cytochrome c reduction assay. Xanthine oxidase (XO, 4 mU/mL) and xanthine (X, 1 mM) were incubated with catalase (CAT) superoxide dismutase (SOD) where indicated. * p < 0.05 X/XO+SOD vs X/XO and vs X/XO+CAT, one-way ANOVA, Tukey post hoc. (b) Time course (0–60 min) of X/XO O2•- generation was measured using HPLC separation of 2-OHE+ and then plotted against the expected O2•- production. (c) Rose Bengal O2•- production rate per light dose. (d) Fluorescent protein (mCherry, tdKillerRed and SuperNova) O2•- production per light dose. Data from (c) and (d) are derived from data presented in Fig. 2. *p < 0.05 SuperNova vs mCherry, ** p < 0.05 SuperNova and tdKillerRed vs mCherry, two-way ANOVA, Bonferroni post hoc. Values are mean ± SD for n = 3 independent experiments.

Article Snippet: SuperNova/pRSETB was a gift from Dr. Takeharu Nagai (Addgene plasmid # 53234) [ 8 ]. mCherry (pmCherry-C1) and tdKillerRed (#FP963, Evrogen) were amplified and ligated into pRSETB using BamHI and EcoRI.

Techniques: Incubation, Derivative Assay

Journal: Free radical biology & medicine

Article Title: Quantification of reactive oxygen species production by the red fluorescent proteins KillerRed, SuperNova and mCherry.

doi: 10.1016/j.freeradbiomed.2019.12.008

Figure Lengend Snippet: (a) Rose Bengal, (b) tdKillerRed, Supernova, mCherry and control (no photosensitizer) were irradiated with equal molar absorptivity at 561 nm in the presence of 0.1 μM SOSG. The initial fluorescence reading (Ex 525 nm; Em 550 nm) was subtracted from the post-illumination reading and presented as the relative fluorescence change. Values are mean ± SD for n = 3 independent experiments.

Article Snippet: SuperNova/pRSETB was a gift from Dr. Takeharu Nagai (Addgene plasmid # 53234) [ 8 ]. mCherry (pmCherry-C1) and tdKillerRed (#FP963, Evrogen) were amplified and ligated into pRSETB using BamHI and EcoRI.

Techniques: Irradiation, Fluorescence

Journal: Free radical biology & medicine

Article Title: Quantification of reactive oxygen species production by the red fluorescent proteins KillerRed, SuperNova and mCherry.

doi: 10.1016/j.freeradbiomed.2019.12.008

Figure Lengend Snippet: Superoxide and singlet oxygen quantum yield of fluorescent proteins relative to tdKillerRed.

Article Snippet: SuperNova/pRSETB was a gift from Dr. Takeharu Nagai (Addgene plasmid # 53234) [ 8 ]. mCherry (pmCherry-C1) and tdKillerRed (#FP963, Evrogen) were amplified and ligated into pRSETB using BamHI and EcoRI.

Techniques: